• Laboratory diagnostics for membranous nephropathy

Autoantibody detection by means of immunofluorescence, ELISA and chemiluminescence

Serological detection of autoantibodies (IgG) against PLA2R or THSD7A, which only requires a blood sample, is easily performed and respresents a non-invasive alternative to biopsies.

EUROIMMUN has developed state-of-the-art test systems based on recombinant antigens for precise determination of autoantibodies. In the indirect immunofluorescence tests Anti-PLA2R IIFT (IgG) and Anti-THSD7A IIFT (IgG), transfected cells which express the antigens on their surface are used as standard substrates. For the enzyme-linked immunosorbent assay Anti-PLA2R ELISA (IgG) the recombinant receptor is biochemically extracted from the transfected cells and then used to coat the microplates, while in the Anti-PLA2R ChLIA (IgG) magnetic particles are coated with the antigens. These methods allow for easy, fast and highly specific detection of autoantibodies in a blood sample.

The immunofluorescence tests are reliable test systems for qualitative detection of autoantibodies against PLA2R and THSD7A. The Anti-PLA2R ELISA (IgG) and the Anti-PLA2R ChLIA (IgG) also allow quantitative determination of the anti-PLA2R antibodies.

Serological detection of autoantibodies against PLA2R and THSD7A
Indirect immunofluorescence test (PLA2R transfected cells, anti-PLA2R positive: left, THSD7A transfected cells, anti-THSD7A positive: right), ELISA (centre)
Serological detection of autoantibodies against PLA2R and THSD7A

Beside its usefulness for differential diagnostics of pMN, the autoantibody titer has a high predictive value with respect to:

  • Disease activity and course
  • Therapy monitoring
  • Risk assessment